Biopharmaceuticals

Bespoke characterization services targeted to your molecule

Biopharmaceutical Testing

Amino acid sequence N-terminal sequencing (Edman chemistry)

Mass-spectrometric sequencing
N-terminal sequencer

On line LC-MS (with MSe and/or MS/MS)

MALDI-MS and MS/MS
Amino acid composition Amino acid analysis Amino acid Analyzer (e.g. RP-HPLC with fluorescence detector)
Terminal amino acid sequence N-terminal sequencing (Edman or mass spectrometry)

Mass spectrometry based sequencing for the C-terminus
N-terminal sequencer

Mass spectrometer capable of providing N- and C-terminal sequence information
Peptide map Peptide mapping On line LC-MS (with MSe and/or MS/MS)
Sulfydryl group(s) and disulfide bridges Non-reduced peptide mapping On line LC-MS (with MSe and/or MS/MS)
Carbohydrate structure Monosaccharide composition analysis

Oligosaccharide population analysis

Linkage analysis

Glycosylation site analysis
GC-MS

LC and on line LC-MS (with MSe and/or MS/MS)

MALDI-MS and MS/MS
Molecular weight or size Intact molecular weight analysis On line LC-MS

MALDI-MS
Isoform pattern IsoElectric Focusing (IEF) Imaged Capillary IEF (icIEF)
Extinction coefficient Optical density measurement and protein concentration determination UV spectrophotometer

Amino acid Analyzer (e.g. RP-HPLC with fluorescence detector)
Electrophoretic analysis Charge and size based electrophoretic analysis icIEF

Capillary Gel Electrophoresis (CE-SDS)
Liquid Chromatography Charge, size and hydrophilicity/hydrophobicity based chromatographic analyses Ion-exchange chromatography (IEX)

Size Exclusion Chromatography (SEC)

Reversed Phase Chromatography (RP-HPLC)
Spectroscopic profiles Secondary and tertiary structure analysis NMR (1D and 2D)

Circular Dichroism (CD)

Fourier Transform-Infra Red (FT-IR)

Fluorescence (Intrinsic and Extrinsic)
Analysis of truncated forms Intact molecular weight analysis

Peptide mapping

Size based chromatographic and electrophoretic analysis
On line LC-MS (with MSe and/or MS/MS)

MALDI-MS

SEC

CE-SDS
Analysis of other modified forms, including Post Translational Modifications (PTMs) Intact molecular weight

Peptide mapping

Oligosaccharide profiling

Isoform profiling
On line LC-MS (with MSe and/or MS/MS)

MALDI-MS

icIEF
Aggregation Size based chromatography, Analytical UltraCentrifugation (AUC) Size Exclusion Chromatography with Multi Angle Laserlight Scattering (SEC-MALS)

Sedimentation Velocity-Analytical UltraCentrifugation (SV-AUC)

Expert assessment of the structure of your biopharmaceutical product.