C-terminal sequencing



There is no fully analogous method akin to Edman chemistry for determining the C-terminal sequencing of a biopharmaceutical. For an assessment of the C-termini, BioPharmaSpec scientists use carboxypeptidase digestion and/or mass-spectrometric peptide mapping strategies.

Intact molecular weight analysis of the product can also be used as an orthogonal procedure to support conclusions regarding the structures of the N- and C-termini.

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