C-terminal sequencing

Definition

There is no fully analogous method akin to Edman chemistry for determining the C-terminal sequencing of a biopharmaceutical. For an assessment of the C-termini, BioPharmaSpec scientists use carboxypeptidase digestion and/or mass-spectrometric peptide mapping strategies.

Intact molecular weight analysis of the product can also be used as an orthogonal procedure to support conclusions regarding the structures of the N- and C-termini.