Peptide mapping definition

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Definition

During peptide mapping, a protein sample is broken down by enzymes into peptides that are amenable to mass spectrometric analysis. The masses of the peptides obtained are then compared to the predicted masses of peptides from a theoretical digest. Comparing the observed data with the theoretical data can be used to confirm the structure of the protein.

Related Content & Resources

Knowledge Center

Scientific Article: Dealing with the challenges of post translational modification (PTMs) Read More
Publication: Foundational Study on Peptide Mapping Using Mass Spectrometry Read More

Case Study

F-star – Peptide Mapping of Bispecific Antibodies

BioPharmaSpec performed on-line Liquid Chromatography with Electrospray-Mass Spectrometric detection (LC/ES-MS, Q-TOF)) of proteolytic digests of F-star bispecific antibody samples to provide informative peptide maps.