PNGase A
Categories:
Glossary: Glycosylation
Definition
PNGase A is an enzyme which cleaves N-linked oligosaccharides from proteins or peptides. It cleaves between the innermost GlcNAc and Asparagine amino-acid resulting in a deaminated protein and free glycan. It cleaves N-linked oligosaccharides from high mannose, hybrid and short complex oligosaccharides such as those found in plant and insect cells.
In contrast to PNGaseF, it will also cleave where a (1,3)-linked core fucose is present.
Related Content & Resources
Case Study
Iksuda Therapeutics – Oligosaccharide Profiling of an ADC
Iksuda required glycosylation analysis for their Antibody Drug Conjugate product to provide functionally important post-translational modification information and to ensure that the chemistry used to attach the drug did not impact glycosylation.
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