PNGase F

Categories: Glossary: Glycosylation

Definition

PNGase F (Peptide:N-glycosidase F) is an enzyme which cleaves almost all N-linked oligosaccharides from proteins or peptides. It cleaves between the innermost GlcNAc (unless it contains a 1,3-fucose) and Asparagine amino-acid resulting in a deaminated protein and free glycan.

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Case Study

European Biosimilar Company – Structural And Physicochemical Characterization And Comparability

Our client is developing a biosimilar to Simponi and required studies including determination of the primary sequence of an EU RMP Golimumab sample and a structural and physicochemical comparability assessment of three EU RMP Golimumab samples.