Glycosylation and Carbohydrate Structure

Comprehensive glycan characterization and site identification

What is Protein Glycosylation?

Attached to asparagine residues Attached to serine/threonine residues
Linear protein consensus sequence No linear protein consensus sequence
10-20 monosaccharide residues in size on average but some variability around this 2-5 monosaccharide residues in size on average but some variability around this
Attached as a large precursor glycan Attached as a single N-acetylgalactosamine monosaccharide
Conserved pentasaccharide core No conserved core – several core structures are possible
Precursor is broken down and remodelled Monosaccharides are added individually to the core N-acetylgalactosamine residue
Contains the monosaccharide mannose Does not contain the monosaccharide mannose

Analyzing Carbohydrate Structure

Monosaccharide composition analysis

Oligosaccharide analysis

Methodology for N-linked Glycan Analysis

Glycosylation analysis: bespoke for your biopharmaceutical