Secondary, tertiary and quaternary structure is often collectively termed as the higher order structure (HOS) of a protein. HOS is responsible for the correct folding and three-dimensional shape of a biopharmaceutical. This can be affected by different formulations, which in turn can affect protein activity. The folding and shape of the protein impacts directly on the functionality of the drug and this correlation is often termed as the “structure function relationship”.
Incorrect higher order structure can also raise safety concerns. If the overall folding and therefore 3D shape of a protein is wrong, receptor binding can be inhibited, immunogenic epitopes can be exposed and aggregation can occur. It is therefore crucial to employ a thorough characterization of the higher order structure of the protein. Biophysical characterization methods should be performed alongside functional analyses and primary structure characterization to allow a full understanding of the overall protein structure.
BioPharmaSpec offers a comprehensive range of biophysical techniques to fully characterize the higher order structure and stability of your protein.